THE EFFECT
OF REEDS AND WASHED RICE WATER ON THE GROWTH AND PRODUCTION OF WHITE OYSTER
MUSHROOM (PLEUROTUS OSTREATUS)
Yusuf L. Limbongan
Universitas Kristen Indonesia Toraja, Sulawesi Selatan, Indonesia
ABSTRACT
This study aims to determine the effect of reeds as a planting medium
and washed rice water on the growth and production of white oyster mushroom.
The research was conducted in April-July 2021 in Polopadang, Kapala Pitu
District, North Toraja Regency.The research was carried out in the form of a
factorial experiment with 2 different factors arranged in a randomized block
design (RBD), namely: As the treatment used Imperata and washed rice water.
Imperata (reeds) as 1st factor consists of three treatment levels, namely R0 =
control, R1 = 10% Reeds, R2 = 20% reeds, while washed rice water as 2nd factor
consists of five treatment levels, namely W0 = control, W1 = 10 ml of washed
rice water, W2 = 20 ml of washed rice water, W3 = 30 washed rice water, W4 = 40
ml of washed rice water. The results showed that the treatment of 10% Imperata
(L1) and 10 ml water leri (L2) gave the best results on the growth and
production of white oyster mushrooms in this case, namely: number of mushroom
pin head, fresh fruit body weight, and mushroom diameter.� Number of pin head and diameter of mushroom
hood have a positive and significant correlation to weight of mushroom, each
with a correlation coefficient 0.799 and 0.807. This research implies that the
use of reeds and rice washing water in the cultivation of white oyster
mushrooms has promising potential. This combination can promote mold growth,
reduce production costs, and contribute to financial sustainability.
Keyword: white
oyster mushroom, growth regulator, washed rice water, reeds.
Corresponding Author: Yusuf
L Limbongan
E-mail: [email protected]
INTRODUCTION
Indonesia is a country that has considerable potential
to develop food and horticultural products. One type of horticultural product
is white oyster mushroom which can be developed and directed to increase
people's income, as well as improve nutritional conditions through
diversification of types of food ingredients. Oyster mushroom is a food
mushroom that comes from the Basidiomycetes group, called oyster mushroom
because its cap is shaped like a circle like an oyster shell (Maulidina et al.,
2015). White oyster mushroom (Pleurotus ostreatus)
contains 9 essential amino acids with a protein content of 19-35% and vitamins
such as B1, B2, niacin, biotin, and vitamin C. In addition, there are minerals
K, P, Ca, Na, Mg and Cu.
Mushroom production in Indonesia in 2016 reached
40,914,331 kg, in 2017 mushroom production decreased to 3,201,956 kg. but in
2018 mushroom production increased again, namely 31,051,571 kg, this was in
line with the increasing demand for mushrooms, considering that mushrooms are
an alternative food that is liked by all levels of society (BPS, 2022). According to reseacrh� currently mushrooms are very popular for
consumption by the wider community, including oyster mushrooms and straw
mushrooms, which have high economic value and are prospective as farmers'
income (Suprapto et al., 2017).
North Toraja is a very potential area for oyster
mushroom cultivation when viewed in terms of climate and temperature and if
viewed from the economic aspect North Toraja is the main tourist destination
after Bali. With so many tourists, the development of restaurants and hotels as
supporting suggestions also increases. Therefore, mushrooms are needed by
hotels and restaurants because tourists who come prefer to consume processed
menus made from mushrooms.
The main medium used in mushroom cultivation is
generally wood sawdust waste which can be obtained from sawing wood. Wood
powder generally used as a growth medium for white oyster mushrooms is sengon
wood powder which is of good quality and contains organic ingredients and
active extracts. Organic materials (lignocellulosic) and active extracts
(resins, tannins) can be used as a medium for fungal growth. Due to the
increasing demand and limitations in nature, alternative media have begun to be
sought as a substitute for sengon sawdust. Reeds are an alternative because
they contain lignocellulose and are abundantly available in the environment as
waste (Puspaningrum, 2013); (Utami &
Susilawati, 2017).
Mushrooms need food in the form of chemical elements
such as nitrogen, phosphorus, sulfur, potassium, carbon which are available in
wood tissue, even in small amounts for life and development (Syawal et al., 2018); (Roshita et al., 2017) and (Girmay et al., 2016). Therefore, external additions are needed, for
example in the form of fertilizer used as a mixed ingredient for making plant
substrates or mushroom growing media (Ishartati et al.,
2017).
Rice is a source of energy and protein, contains
various elements of minerals and vitamins. Washed rice water is also easy to
obtain because most Indonesian people use rice as a staple food. Washed rice water
is water used for washing rice which has not been widely used by the community.
This is because the community does not know the benefits of washed rice water.
Washed rice water contains the elements N, P, K, C and other elements (Suprapto et al., 2017). Mushrooms need carbon, nitrogen, vitamins, and
minerals for their growth. Types of vitamins that are very necessary for the
growth of oyster mushrooms are thiamin (vitamin B1), nicotinic acid (vitamin
B3), pantothenic amino acid (vitamin B5), biotin (vitamin B7), pyrrodoxin, and
inositol.
The aim of the study was to determine the effect of
the composition of the reeds in the growing medium and the dosage of washed
rice water as a growing medium on the growth and production of oyster mushrooms
as well as to determine the composition of the reeds and the dosage of washed
rice water which affected the growth and production of white oyster mushroom.
Based on the background, a study will be carried out
to determine the effect of reeds in growing media and washed rice water on the
growth and production of white oyster mushrooms.
METHOD
Time and Place of Research
This research was
conducted in Polopadang, Kapalapitu, North Toraja Regency. This research takes
place from April-July 2021.
Materials and tools
1. The materials used in this study were oyster mushroom
seeds, sawdust, reeds, rice bran, dolomite lime, milled corn, clean water,
alcohol, cotton, paper and spirits.
2. The tools used in this study were clear plastic,
(17X35cm), machetes, paralon pipes, rubber bands, drums, gas stoves, sprayers,
litmus paper, scalpel, Bunsen burner, scales, sigmat, ruler and knife.
Research methods
This research was
conducted using a factorial randomized block design (RBD) with two factors. The
first factor is reeds and the second factor is washed rice water.
|
�Imperata/
Reeds : |
Washed rice water : |
|
�R0 =
control |
W0 = control |
|
�R1 =
10% reeds |
W1 = 10 ml washed rice
water |
|
�R2 =
20% reeds����������� |
W2 = 20 ml washed rice
water |
|
|
W3 = 30 ml washed rice
water |
|
|
W4 = 40 ml washed rice
water |
So there were 15 treatment combinations, each repeated 4
times � 60 plots and 240 baglogs.
Preparation of Planting Media
Basic media: 200 g of bran, 200 g of corn, 16 g of
dolomitic lime
R0W0 : basic media 1000 g sawdust
R0W1 : basic medium + 20 ml washed rice water
R0W2 : basic medium + 40 ml washed rice water
R0W3 : basic medium + 60 ml washed rice water
R0W4 : basic medium + 80 ml washed rice water
R1W0 : basic medium + 875 g sawdust + 125 g reeds
R1W1 : basic medium + 875 g sawdust + 125 g reeds + 10 ml
washed rice water
R1W2 : basic medium + 875 g sawdust + 125 g reeds + 20 ml
washed rice water
R1W3 : basic medium + 875 g sawdust + 125 g reeds + 30 ml
washed rice water
R1W4 : basic medium + 875 g sawdust + 125 g reeds + 40 ml
washed rice water
R2W0 : basic medium + 750 g sawdust + 250 g reeds
R2W1 : basic medium + 750 g sawdust + 250 g reeds + 10 ml
washed rice water
R2W2 : basic medium + 750 g sawdust + 250 g reeds + 20 ml
washed rice water
R2W3 : basic medium + 750 g sawdust + 250 g reeds + 30 ml
washed rice water
R2W4 : basic medium + 750 g sawdust + 250 g reeds + 40 ml
washed rice water
Before planting
(inoculation) of seedlings into the planting medium, the following preparations
are made:
Prepare tools and materials to be used.
Before sawdust is
used, sawdust is sifted first to get fine powder. After that the sawdust is
washed to reduce the sap content in the sawdust because wood sap can suppress
mycelium growth. Then the reeds were cut into pieces with a size of
approximately 5-8 cm. Mix sawdust with other ingredients such as rice bran,
dolomite lime, corn flour and reed leaves to be used according to the
treatment, add about 50-60% of the amount of the ingredients, mix the
ingredients until completely smooth and smooth so that it is easy clenched.
Composting needs to
be done beforehand, so that it can decompose into simpler compounds that are
easily digested by oyster mushrooms. The composting process is carried out by
covering the tarpaulin dough for 4 days.�
Putting the media that has been mixed into a clear plastic bag (baglog)
and compacting it then the top of the plastic bag is given a paralon ring and
covered with newspaper and then covered with plastic and tied with a rubber
band. Furthermore baglog sterilization, wet sterilization using drums. The
baglog wet sterilization process is carried out by steaming the media at 100�C
using a furnace. Sterilization lasts for 8-10 hours, it is hoped that the
intruding microorganisms can be suppressed.
Cultivating Oyster Mushrooms
After the oyster
mushroom planting media has been cooled and sterilized both the materials and
tools used, planting is done by removing the seeds from the bottle using a
scalpel then the mushroom seeds are planted into the growing media (baglog)
after that it is covered using paper, this process is very important because
where must maintain sterility because oyster mushroom seeds are easily
contaminated.
Incubation
Incubation is the
storage stage of baglog which has been inoculated into the incubation chamber
until all of the baglog is covered with white mycelium. The incubation period
usually lasts 30 days. Certain arrangements should be made so that the mushroom
mycelium can grow quickly. Things that must be arranged in the incubation room
include the room and incubation place must be clean from contaminants, dry,
(with humidity 60%) good air circulation, should not be exposed to direct
sunlight, and room temperature is maintained at 28-30�C. During this incubation
period routine monitoring must be carried out, during this incubation period
problems in the form of contamination and pest attacks usually begin to arise.
Contaminated media must be separated and discarded immediately so as not to
infect other baglogs.
Mushroom Maintenance
The maintenance
that must be considered is the temperature required in the range of 23-28�C,
with the optimum air temperature at 25�C. Maintenance can be done by watering
jamjur 3 times a day.� To avoid pests and
diseases in mushroom cultivation, it is carried out from the start of making
media, places or locations with sterile conditions or locations that are clean
from contaminants such as insects, rodents, microbes and harmful compounds.
Harvest Oyster Mushrooms
White oyster
mushrooms are harvested when the growth of the fruiting bodies has been
maximized. This growth period is marked by the maximum and perfect size and
shape of the fruit body. The most appropriate harvest time is 4-5 days from the
formation of the fruit body candidates
Observational Variables
Observations were
made on the growth of oyster mushrooms which include:
1. Number of fruiting bodies, counting the number of
fruiting bodies formed is carried out at harvest time with an interval of 2
weeks until crop production decreases.
2. The wet weight of the mushroom fruiting bodies (g), was
weighed at each harvest with an interval of 2 weeks until the harvest
production decreased.
3. Diameter of the fruit cap (cm), measurement of the fruit
cap is carried out at harvest time with an interval of 2 weeks until crop
production decreases.
Data analysis
Data were analyzed
using variance (ANOVA) and if it had a significant effect it would be continued
with the Least Significant Difference (LSD) test at 5% level.
RESULTS AND DISCUSSION
Analysis of Variance number of pin head, diameter of
mushroom hood and weight of mushroom (Table 1), showed that the application of
reeds had no significant effect on the variable number of pin head, diameter of
mushroom hood and weight of mushroom.
This is due to the insufficient availability of nutrients
namely cellulose and lignin in Imperata so that they are less able to support
further fungal growth as indicated by the addition of cap diameter which was
not significantly different for each level of treatment. This is because the
reeds contain cellulose, hemicellulose and lignin. For optimum growth of
mushrooms requires a number of nutrients obtained from the planting medium.
The results of lignocellulosic analysis showed that reeds
had higher cellulose than sawdust of sengon wood. Cellulose is a group of
polysaccharides that will be broken down into monosaccharide groups, namely
glucose which serves as a carbon source which is a macro element as a
constituent of the structure of fungal cells (Mudakir & Hastuti,
2015). Lignin is degraded into glucose and other compounds,
glucose and these compounds are used as energy reserve nutrients to produce
optimal fresh fruit (Nurafles, 2015). The correct composition of lignin and cellulose in the
combined treatment of sawdust of sengon wood and reeds is urgently needed to
obtain maximum growth of white oyster mushrooms (Fatmawati, 2017); (Ali et al., 2023);
(Tafzi et al., 2021).
Table 1. Analysis of Variance for number of pin head,
diameter of mushroom hood and weight of
mushroom
|
Source of Variation |
Number of
pin head |
Diameter of
mushroom hood |
Weight of
mushroom |
|||
|
Block |
1.44 |
ns |
3.16 |
* |
2.22 |
ns |
|
Treatment |
7.27 |
** |
5.42 |
** |
11.81 |
** |
|
Reeds (R) |
0.05 |
ns |
0.04 |
ns |
0.01 |
ns |
|
Washed rice water (W) |
16.99 |
** |
14.08 |
** |
23.44 |
** |
|
Interaction (R x W) |
4.02 |
** |
2.30 |
* |
8.91 |
** |
Description: **=
significant at F 1%, *= significant at F 5 %, ns = non significant
The results of the analysis of variance on the total number of fruit
showed that the rice washing water had a very significant effect. This is
presumably because rice washing water contains nutrients, carbohydrates,
protein, minerals and vitamins needed for mushroom growth (Igile et al., 2020); (Oktavianus Mandi, n.d.). High
carbohydrates can form the hormones auxin and gibberellin which function as a
stimulant for shoot growth , emergence of shoots and stimulate root growth (Wahidah & Saputra, 2015).
The results of the LSD test on the total number of fruiting bodies gave
the best results in the administration of rice washing water (W2) 20 ml per
baglog, which was significantly different from the other treatments (Table 2).
This is presumably because baglog requires more nutrients for mushroom growth.
The results of the analysis of variance on the total fruit bodies showed
that the administration of rice washing water (W2) had a very significant
effect. This is presumably because rice washing water contains vitamin B1 as a
form of auxin hormone, which when this auxin hormone is combined with cytokinin
hormones can induce budding, besides auxin leri water also contains gibberellin
hormone which can induce dormant buds.
The results of the test of variance showed that giving 20 ml of rice
washing water (W2) per baglog produced total fresh fruit bodies (122.78 g),
which was significantly different from the treatment without rice washing
water. This is presumably because baglog requires more nutrients for mushroom
growth.
The results of the
LSD test on the length of the mushroom stalks showed that the provision of rice
washing water had a significant effect, presumably because the nutrients needed
must be sufficient and should not be too moist. The main factors in mushroom
stalk length are environmental factors, especially temperature and humidity.
The water content in the media greatly influences the growth and development of
the mushroom mycelium. Too little water will result in growth and development
being disrupted, even stopping altogether. However, if there is too much water,
the mycelium will rot and die. Mushrooms grow well in moist conditions, but do not want standing water (P, 2017). Mushrooms that
are too dry are of low quality due to insufficient temperature and humidity so
that the mushroom stalks are longer and thinner (Normalia Lambe�Toding, n.d.).
Table 2. Number of Pin Head, Diameter of Mushroom Hood, and Weight
of Mushroom
|
Treatment |
Number of pin head |
Diameter of mushroom hood (mm) |
Weight of mushroom (g/baglog) |
|||
|
R0 W0 |
15.67 |
a |
9.87 |
a |
205.43 |
a |
|
R0 W1 |
16.33 |
ab |
10.17 |
a |
355.90 |
bc |
|
R0 W2 |
28.67 |
c |
16.00 |
c |
541.87 |
e |
|
R0 W3 |
16.00 |
a |
10.87 |
a |
352.53 |
bc |
|
R0 W4 |
17.67 |
ab |
10.47 |
a |
430.30 |
cd |
|
R1 W0 |
19.67 |
ab |
10.87 |
a |
352.77 |
b |
|
R1 W1 |
16.67 |
ab |
10.47 |
a |
334.90 |
b |
|
R1 W2 |
21.67 |
b |
12.93 |
b |
492.10 |
de |
|
R1 W3 |
16.00 |
a |
10.83 |
a |
366.33 |
bc |
|
R1 W4 |
15.67 |
a |
10.43 |
a |
349.37 |
b |
|
R2 W0 |
16.00 |
a |
10.33 |
a |
332.50 |
b |
|
R2 W1 |
15.33 |
a |
10.23 |
a |
353.43 |
bc |
|
R2 W2 |
21.33 |
b |
12.33 |
b |
385.30 |
bc |
|
R2 W3 |
20.67 |
ab |
11.70 |
ab |
458.87 |
de |
|
R2 W4 |
16.00 |
a |
10.73 |
a |
326.80 |
b |
|
LSD 5% |
5.28 |
2.63 |
90.56 |
|||
Description:
The average value followed by the same letter in the column and the interaction
is not significantly different at the 5% LSD test level.
The
results of observations on the total fruit body weight and its variance in the
table 1 show that the application of Imperata and rice washing water has a very
significant effect on fruit body weight. The results of the 5% LSD test on
total fruit body weight showed that 10% Imperata (R1) application resulted in a
total fruit body weight significantly different from other treatments. The best
rice washing water (W2) was 20 ml per baglog resulting in a total fruit weight
significantly different from other treatments. The results of observing the
diameter of the mushroom caps at harvest I and their variances indicating that
Imperata and the interaction between rice washing water had a very significant
effect on the diameter of the mushroom caps.
The results of the 5% LSD test on the diameter of the mushroom showed
that the application of 10% Imperata (R1) produced the widest fruit cap and the
application of 20 ml rice washing water (W2) produced the widest diameter which
was different significant with other treatments. Analysis of variance on the
variable number of pin head, diameter of mushroom hood and weight of mushroom
shows that the interaction between the provision of reeds and rice washing
water has a very significant effect on the number of pin head, and weight of
mushrooms and has a significant effect to the variable diameter of mushroom
hood. This is in line with the results of (Simorangkir
et al., 2018); (Lavenia
et al., 2021) and (Nasution
et al., 2022).
The LSD test at 5% test level (Table 2)
shows that the R0W2 treatment combination produced the highest number of pin
heads, significantly different from all other treatment combinations. The R0W2
treatment combination also produced the largest mushroom head diameter which
was significantly different from all other treatment combinations. Treatment
R0W2 produced the highest weight of mushrooms, but not significantly different
from R1W2 and R2W3, but significantly different from the other treatment
combinations. This shows that the addition of reeds in the mushroom medium is
not suitable for the growth of white oyster mushrooms, especially in the
variable number of pin heads, diameter of moshroom hood and mushroom weight (Puadi et
al., 2022); (Wati
& Yuliani, 2012); (Naila
& Purnomo, 2016).
Table
3. Correlation Analysis Between Number of Pin Head,
Diameter
of Mushroom Hood and Weight of Mushroom
|
Variable |
Number of
pin head |
Diameter of
mushroom hood |
Weight of
mushroom |
|
Number
of pin head |
1 |
||
|
Diameter
of mushroom hood |
0.960 ** |
1 |
|
|
Weight
of mushroom |
0.799 ** |
0.807 ** |
1 |
Description:
** significant pada r 1%.
Mushroom
need food in the form of chemical elements such as nitrogen, phosphorus,
sulfur, potassium, carbon which are available in wood tissue, even in small
amounts for life and development (Kalsum
et al., 2011); (Pribady
et al., 2018); (Rochman,
2018). The amount of mushroom fruit body was
affected by the absorption of nutrients in the media (Wahidah & Saputra, 2015); (Khan et
al., 2013); (Alfarizi
et al., 2021) and (Simorangkir
et al., 2018).
Correlation analysis between number of pin head, diameter of mushroom
hood and weight of mushroom is presented in Table 3. Table 3 shows that number
of pin head and diameter of mushroom hood have a positive and significant
correlation to weight of mushroom, each with a correlation coefficient 0.799
and 0.807. This means that the more the number of pin heads and the diameter of
the mushroom head, the higher the weight of the mushroom. The number of pin
heads has a positive and significant correlation with the diameter of the
mushroom hood, meaning that the greater the number of pin heads, the greater
the diameter of the moshroom hood. The results of this study are in line with
the results of previous studies that the number of pin heads is directly
related to the diameter of the mushroom hood (Girmay
et al., 2016), as well as the weight of the mushroom
fruit bodies (Nasution
et al., 2022).
CONCLUSION
Based on the results of the research
that has been done, it can be concluded that the application of reeds and rice
washing water has a different response by white oyster mushroom plants. The
composition of 10% reeds and 90% basic medium responded well by white oyster
mushrooms, especially in the total number of pin heads, total fresh fruit body
weight, and mushroom cap diameter. The interaction between 20 ml rice washing
water and 10% reeds + 90% basic medium had a good effect on the total number of
pin heads, total fresh fruit body weight, and mushroom cap diameter.� Number of pin head and diameter of mushroom
hood have a positive and significant correlation to weight of mushroom, each
with a correlation coefficient 0.799 and 0.807.
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